Objective: to characterizeand prepare pomegranate extract and study its antioxidant, anti-cancer activity, determine the total phenols and flavonoidsdetermine the active ingredients using LGC MAS technology. Materials and Methods: Preparation of Punicagranatum extract in distilled water and alkaline energy water, and characterization of the ingredients in each extract was carried out using GC-MASS spectroscopy; cytotoxicity was measured against MCF-7 cancer cell line. Results: it was found that alkaline water extract showed the highest concentration of phenols and flavonoids at a concentration of 34.32 and 59.65μg/g and the percentage of the two compounds in the GC-MASS assay Hexadecanoic acid, methyl ester, 11- Octadecanoic acid, methyl ester. In alkaline water with the highest concentration (24%), 15.34%) compared to distilled water extract Punicagranatum, as it was found to be 25.67 and 39.22, noting that these two compounds are anti-cancer compounds. Furthermore, the study proved that Punicagranatum alkaline extract showed a high inhibitory activity for a type of cancer breast cancer cells line MCF-7. Conclusions: MCF-7 cell proliferation were significantly decreased with a concentration-dependent response, and it was found that a concentration of 0.5 µg/ml gave an inhibition rate of 93.47%, and Punicagranatum extract with alkaline water showed a high effectiveness in inhibiting the following microorganisms: S. aureus B. cereus E. coli S. typhimureum A. niger C. albicans, inhibition and antioxidant activity 87.18 at a concentration of 40mg/ml, the concentration of the ingredients are more in alkaline energy water extract than distilled water extract. Keywords: Punicagranatum extract, alkaline water, phenols, flavonoids.