Background: Due to their high mineral and vitamin content, beetroot and onions, which are frequently utilized as antibiotics, antioxidants, and nutrients, are generating growing interest in their potential medical applications. Objective: The current study's is to separate the active components from beetroot and onions and use them as antimicrobial agents. Materials and Methods: A mixture of beetroot and onion in varying ratios, as well as fungal and bacterial cultures, were created for testing. Various procedures, including hot water, were utilized to extract the active components. Results: According to the findings, hot alcohol was used to extract the components that were the most active. Depending on the concentration of the active ingredients in the extract solution, the extracts exhibit varying bioactivities. Conclusions: Extracts of beetroot and onions can be used to treat Candida albicans. Conclusions: The antifungals nystatin, miconazol, and clotremazol as well as beetroot and onion demonstrated the best efficacy against Candida albicans at 95/5, 50/50, and 75/25, respectively.