The condition of obesity is a persistent ailment characterized by the atypical or excessive buildup of adipose tissue, resulting in compromised well-being and an elevated likelihood of morbidity and chronic health issues. The global incidence of individuals with obesity and overweight conditions has been on the rise for numerous decades. The goal of the study is to identify the relationship between body mass index (BMI), insulin, transferrin, iron, and lipid profile in obese males. Ninety males were recruited into the study; forty participants were assigned to the obese group were have BMI>30 kg/m2, twenty to overweight group were have BMI between 25-30 kg/m2, and another thirty to the control group were have slow BMI<25 kg/m2. The serum Transferrin, serum fasting insulin, serum iron, lipid profile and fasting glucose was evaluated in each of studied groups. The Obese group showed significant increase of the serum insulin concentration compared with the control group (16.77±7.68 vs. 11.7 ±2.79, p <0.001). As well as in transferrin and lipid profile significant change was detected. No prominent differences were detected in the three groups in iron and glucose concentration. Investigation of serum transferrin can be participated in future as iron deficiency marker in obese subject in conjugation with serum iron and serum ferritin.