This study aims to test and analyse the effect of Work-Life Balance on Job Satisfaction with Work Motivation as mediation for lecturers working in Universities in West Kalimantan. The research approach used in this research is quantitative research. The method used is a survey method by distributing questionnaires to 100 lecturers in West Kalimantan; the data is then processed by the Structural Equation Model (SEM) method. The results of data analysis were carried out with the help of Smart Pls 4.0. The results obtained are work-life balance affects work motivation; work motivation affects job satisfaction while work-life balance affects job satisfaction when fully mediated by work motivation.