Background: The staff nurses services of tracheostomy patients care play a very vital role based on the principle of ’time saving is lifesaving’. Tracheostomy care is a major health issue in various parts of the world. The number of patients with tracheostomy is increasing in both developed and developing countries. This study was conducted with the aim of investigating the effectiveness of video assisted teaching on knowledge and practice regarding tracheostomy care among the staff nurses and to develop protocol on tracheostomy care. Methods: A pre-experimental research approach, one group pre-test and post-test research design was adopted to carry out the present study non probability convenient sampling technique was used to select 30 staff nurses. Data was collected by means of structured knowledge of questionnaire to assess the knowledge and observational checklist to assess the practice of staff nurses regarding tracheostomy care. The analysis was done using descriptive and inferential statistics in terms of frequency, percentage, means, median, mode, standard deviation, mean difference t-test and chi square test in IBM SPSS Statistics 20. The significance level was set at P≤0.05. Results: A purely improvement in the score of knowledge and practice regarding tracheostomy care among staff nurses was observed (P<0.05). There was also a significant association between the knowledge score and the clinical experience (P= 0.03) and CNE on tracheostomy care (P= 0.01). Conclusion: The staff nurse has inadequate knowledge and fair practices regarding tracheostomy care. A video assisted teaching and protocol administration is an effective strategy to increase their knowledge and improve their practice. Also, holding in-service training courses regarding the tracheostomy care is considered necessary.