Despite a global immunization campaign, hepatitis B virus (HBV) infection remains a severe public health risk. The study advances our understanding of the diversity and evolution of the hepatitis B virus by studying virus genotypes using PCR and sequencing. This insight will be extremely useful in the development of targeted medicines and immunizations to combat HBV infection. The results of HBV testing on twenty samples, three of which tested positive and the remaining 17 were negative. A fast test, however, yielded positive results, indicating a mutation that hampers diagnosis in the detection of a 492 base pair band. The HBV sequences (seq1) were observed to mimic strains from other countries, with four SNPs causing a gap between seqs 1 and 2. It was also stated how many people in China who donated blood tested positive for HBV DNA. According to the phylogenetic tree analysis, sequences 1 and 2 were 40% related to one another in an international strain, whereas sequences 3 and 4 were independently classified and shared 41% similarity, implying that just a fraction.