The process of collecting and identifying Staphylococcus aureus bacteria from nasal swab samples obtained from 20 horses at the Equestrian Club in Baghdad Governorate and the Horse Recreation Club in Babylon Governorate was conducted between October 2022 and March 2023. Identification of the isolates was carried out by observing colony morphology on various bacterial media, performing gram staining, and conducting biochemical tests, notably the catalase test. Antimicrobial susceptibility testing was also performed to determine the resistance profile of S. aureus. Out of the total nasal swab samples collected, S. aureus was detected in 12%, while other types of bacteria accounted for 8% of the samples. Confirmation of Staphylococcus samples was achieved through Gram staining and culturing on selective medium, such as Mannitol Salt Agar, which produced characteristic yellow colonies. Additionally, the catalase test yielded positive results, indicated by the production of bubbles, confirming the presence of the catalase enzyme. To assess the virulence factors and pathogenicity of the bacteria, they were cultured on Gonco Red Agar medium to evaluate biofilm formation, resulting in a positive outcome. Susceptibility testing revealed varying degrees of susceptibility among S. aureus strains to antimicrobial agents, with Ciprofloxacin (34%), Trimethoprim (20%), Amikacin (11%), and Tobramycin (18%) showing effectiveness. Conversely, S. aureus exhibited 100% resistance to Gentamicin and Ampicillin.