Background: Domestic dove, often known as city dove or rock dove, is a subspecies of the rock dove. Domesticated birds began with dove. The massive buzzard (Buteo buteo) inhabits in many ecosystems across the globe. The Accipitridae family and Buteo genus include it. Buzzards hunt and eat alone, however they may gather in flocks during migration or when food is plentiful. Aim to study: The aim of this research to comparative physiological study of pancreas in collared dove (Sterptopelia decaota) and Buzzard (beuteo vulpinus). Methods: This research employed 10 healthy adult Dove (Streptopelia decaocto) and buzzard (Beuteo beuteo vulpinus) per feeding method. Babylonian tradesmen traded birds. These birds of each species for used on the physiological studies. Results: Mean insulin, glucagon, and somatostatin levels in Buzzard and Dove were compared, and the findings indicated a substantial difference. Buzzard had a higher mean insulin level difference (603.59 ± 25.0) than Dove (414.73 ± 29.2). However, buzzard had a higher mean difference in glucagon levels (373.80 ± 16.3) compared to Dove (303.65 ± 32.3). Additionally, Buzzard had a higher mean difference in somatostatin levels (454.89 ± 24.1) compared to Dove (368.80 ± 28.6). Conclusion: The results showed that insulin, glucagon, and somatostatin were all significantly different from one another.