This study is part of a doctoral thesis aimed at developing and validating a theoretical-based model to investigate factors influencing utilisation of Research4Life databases in National Agricultural Research Institutes (NARIs) in Nigeria. Research4Life databases are underutilised in the NARIs in spite of their importance in agricultural research. To enhance their utilisation, an appropriate model was required. This study developed and validated the Utilisation of Research4Life Database Model (URDM) made up of 22 independent constructs. Some constructs from Diffusion of Innovation; Technology Acceptance Model; Unified Theory of Acceptance and Use of Technology, and Technology, Organisation and Environmental models were adopted and others introduced to develop the model. To validate the model, the study adopted the descriptive survey design of the correlational type. Simple and stratified random sampling techniques were used to select 744 (62%) scientists from a population of 1,205. A validated and pre-tested questionnaire consisting of 105 questions arranged in five sections A – E with 0.99 reliability coefficient based on Cronbach alpha method was used for data collection. Simple percentage, mean, correlation, multiple regression and analysis of variance were used to analyse the data. All the 23 formulated hypotheses were significant. The 22 independent factors showed direct positive relationship with utilisation of the databases. Overall, the URDM accounted for 67% level of utilisation of Research4Life databases in NARIs. Thus, the model is adequate for investigating factors influencing utilisation of Research4Life databases in Nigeria. The findings of this study contributed to the literature by validating the URDM in NARIs in Nigeria.