The research was held in Kailali District of Nepal to assess the effect of different packaging materials on shelf life and quality of banana fruit. The experimental set up was done in Completely Randomized Design (CRD) with five treatments of different packaging, each replicated four times. There were five different treatments used for the study and they were allotted as follows: T1: No packaging/open (control) T2: Banana leaf, T3: Straw, T4: Polythene bag, T5: Cardboard box. A digital hygrometer for measuring relative humidity and thermometer for measuring temperature were used. RH was recorded every 3rd day. Similarly, temperature was also recorded during those days. R-Studio 4.2.2 version was used for data analysis. TSS was found highest in polythene bag followed by cardboard box and lowest was control followed by straw. TSS (16.020 Brix) was found in polythene bag at 13th day and lowest TSS (5.250 Brix) in at 1st day. At 16th day, maximum TSS was found in polythene bag (15.320 Brix) and minimum in control (12.720 Brix). Titrable Acidity (TA) tends to increase at initial stage but later decreases with increase in storage time. The highest TA (0.32%) was found in polythene bag and lowest (0.24%) was found in control at day 16th. Physiological Loss in Weigh t(PLW) tends to increases with increase in storage time. The highest PLW (27.68 gm) was found in Control and lowest loss (21.61 gm) was found in polythene bag at day 16th. The firmness was decreased with the advancement in storage time in all treatments. The highest firmness (9.385kgcm-2) was found in polythene bag at 1st day and lowest (9.12 kgcm-2) was found in control at 1st day. At the end of the day i.e., at day 16th, maximum firmness was observed in Polythene bag (3.34 kgcm-2), and minimum was observed in control (2.07 kgcm-2) and pulp thickness tends to increase with increase in storage time. At day 16th, the highest pulp thickness (3.34 cm) was found on banana leaf and lowest (2.07 cm) on straw. Based on these findings, it is evident that packaging bananas in polythene bags resulted in significant improvements in weight loss, TA, firmness, TSS, pulp thickness, and peel thickness. This suggests that using polythene bags as packaging material provides better quality and extends the shelf life of bananas compared to the other materials tested in the study.