Non-starch polysaccharides (NSPs) are not fully digested by broiler’s endogenous enzymes and consequently the soluble NSPs in feed results in high digesta viscosity and poor retention of nutrients. Supplementation of NSPs digesting enzymes may release the nutrients from feed and reduce the anti-nutritional effects of NSPs. This study was executed to investigate the effect of NSPs digesting enzymes (Endo-Power) multi enzyme supplementation levels (500g/ton of feed) with standard diet (SD) on immune response, blood biochemistry and growth performance of broilers chickens during 1–35 days of age. A total of 200 unsexed 1-day-old Ross308 broiler chicks were randomly distributed, to four treatments. There were fifty chicks per treatments. Results showed that diet with multi enzyme supplementation significantly increased the humoral immune response against NDV of broilers. Addition of multienzymes at levels (500g/ton of feed) significantly increased body weight gain BWG and improved Feed consumption ratio FCR, compared to the control treatments. Alanine aminotransferase (ALT) and aspirate aminotransferase (AST) were significantly higher for treatments without multi enzyme than the treatments with multi enzyme. Supplementation of multienzymes significantly decreased ALT and AST compared to the control group.