In this study, the presence of Trypanosoma evansi was detected and identified humans in Babylon province in Iraq by blood smears and confirmed by polymerase chain reaction (PCR). Try this study by taking a sample blood from a camel breeders (humans) by Venipuncture from north and south of Babylon. 75 blood samples were collected from humans, adult and young of all sexes. The blood smears result was in humans 0% (0/75). The infection rate using PCR technology was 12% (9/75). This was the first study in Iraq to reveal the presence of the disease. Trypanosoma evansi in camel breeders (humans).