The present study was aimed to isolation and identification Candida species from different clinical cases and to determine antifungal resistance. A total of 116 samples were collected from various clinical cases, including 41 oral swabs from children with oral thrush attending Tikrit Maternity and Paediatrics Teaching Hospital in Tikrit, 32 vaginal swabs from women with vaginal candidiasis, and 43 urine samples from individuals with urinary tract infection. The morphological and biochemical testing revealed that 16 (45.7%) isolates of Candida spp. were found in the oral samples and were determined to be pathogenic, 10 (31.1%) from vaginal swabs, 14 isolates (36%) from urine samples, C.tropicalis pathogenic samples that 6(17.3%) isolate from oral samples, 7 (21.8 %) from vaginal swabs, 10 isolates (26%) from urine samples, C.parapsilosis pathogenic samples that 5 (14.2%) isolate from oral samples, 8 (25.3%) from vaginal swabs, 13 isolates (33%) from urine samples. While C.krusei pathogenic samples that 8 (22.8%) isolate from oral samples, 7 (21.8%) from vaginal swabs, and 2 isolates (5%) from urine samples. Agar well diffusion method results revealed that the Rezafungin was the most effective antifngal which inhibited 87.9%.