Puskesmas (Community Health Center) are workplaces that have occupational safety and health risks to health workers. The level of occupational safety and health risks can be significantly minimized by implementing safety behaviors. This study aims to determine the effect of OSH training (Occupational Health and Safety) based on the introduction of potential hazards to the safety behavior of officers. An observational study was conducted to identify potential hazards as training material as well as quasi-experimental experiments on 20 health workers who took injection actions at the Puskesmas in February - April 2020. Data were collected using an observation checklist sheet. There are 5 potential hazards in the low-risk category, 12 potential moderate risks and 18 potential high-risk hazards in the Wolasi puskesmas. Furthermore, there was a statistically significant effect between interventions before and after the intervention in terms of the use of personal protective equipment (p = 0,000) and the use of equipment and equipment (p = 0.003). While there was no statistically significant effect between intervention and after the intervention in terms of the respondent's work position (p = 0.164). According to the results of this study, training based on the introduction of potential hazards increases officer safety behavior.