Klebsiella pneumoniae is a mainly cause of severe respiratory infections which may leading to a chronic inflammation or to death, Azithromycin has recently received a lot of scientific attention due to its outstanding effectiveness in treating Other bacterial and some parasitic infections, because it is widely and safe for human use and is believed to be associated with side effects fewer compared with the other macrolide. The current study aims to isolation K. pneumonia from respiratory infection and evaluation histological changes on lungs and possible protective role by azithromycin and of VIT. C, included isolation K.pneumenia from diagnosed patients with respiratory diseases from Samarra hospital at January to April 2024 .All sputum samples were cultured by using selective media for K.pneumonia and Vitic device. In this study used 15 mice which randomly divided to three groups: The first group no treatment, second group treated with 30mg/kg of azithromycin, while third group treated with 30mg/kg from azithromycin plus 20mg/kg of vitamin C. In the results included morphologic the microscopic and biochemical examinations to identify of klebsiella and the histological changes of the lung tissue which included hemolysis in different area of tissue, thickening of the alveoli walls and infiltration of neutrophil and inflammation cells that showed in sections of groups which treated with k.pneumonia, while azithromycin and vitamin were worked on repair abnormalities and the pathologic changes on lung tissue.