The Objective of this research to convert sulfamethoxazole to nano-sulfamethoxazole by Sol-gel method and prepare a pharmaceutical formulation of sulfamethaxazole, prepared at a concentration of 0.25% and in the form of a spray to treat fungal and bacterial infections resulting from wounds of skin, its good for heath. The nano particles characterized by powder X-ray Diffraction (PXRD), atomic force microscope (AFM), Scanning electron microscopy (SEM). The results showed successful that nano sphere sulfamethoxazole and it size between (37-55) nm, all these tests showed different type of nanostructures such as nanotubes and nanoparticles, other biological test MIC & MBC show superior biological activity. The stability of the prepared formula was studied for 6 months under temperature conditions (30, 40) degrees Celsius and relative humidity (70, 75) controlled, which showed the stability and stability of the composition. An experiment was also conducted on laboratory animals (mice) by making a wound in them and treating with the prepared formula with a comparison with the normal non-nanostructure.