Zainacin Dadin duniya is known for its antioxidant and liver-protecting properties. This research assesses the phytochemical characteristics and impact of Zainacin Dadin Duniya water extract on growth and hematological factors in Wister rats with acetaminophen-induced liver damage. 60 female and 30 male rats were divided into five groups with each group consisting of 18 rats selected randomly. Group 1 received saline, group 2 received a liver medicine, group 3 received acetaminophen, group 4 received a daily dose of aqueous extract at 500 mg/kg, and group 5 received a daily dose of aqueous extract at 500 mg/kg along with 3 ml/day of acetaminophen, all administered for 28 days. The animals were weighed before and following the experiment. The hematological parameters were determined, including packed Cell Volume (PCV), Red blood Cell (RBC), Haemoglobin (Hb), Mean Cell Volume (MCV), and White Blood Cell (WBC). Findings indicated that the extract contains a considerable quantity of phytochemicals. Findings indicated a notable weight gain (P<0.05) in Groups 1, 2, 4, and 5 and weight reduction in Group 3. In Group 3, there was a significant decrease in erythrocyte counts (4.29%), packed cell volume (33.00%), and hemoglobin (6.17%) values, while there was an increase in leukocyte (9.62%) and Mean Cell Volume (54.33%) values compared to the other groups. The presence of acetaminophen in the treatment given to Group 3 rats could lead to a decline in their growth performance and trigger adverse reactions in their blood parameters, potentially impacting their overall development. The segment may act as a strong remedy for acetaminophen-induced hepatocytes by blocking those reactive oxygen species.