This study aimed to assess the knowledge and practices related to the prevention of anemia among adolescent girls at a selected nursing college in Bengaluru. Anemia, a significant public health concern, especially for adolescent girls, can lead to serious health issues. The study objectives included evaluating the level of knowledge about anemia prevention, understanding the practical measures adopted, and analyzing the association between these factors and various demographic variables. A descriptive research design was used, and data were collected from 50 participants through a structured questionnaire. The results indicated that 70% of the participants had an adequate understanding of anemia prevention, while 14% had moderate knowledge, and 16% demonstrated inadequate knowledge. In terms of practical application, only 8% of the participants practiced good preventive measures, with 46% showing moderate practices and 46% displaying poor practices. Statistical analysis revealed significant associations between knowledge levels and certain demographic factors, such as type of family and dietary habits, while no significant relationship was observed between practical measures and socio-demographic factors. The findings underscore the need for improved education and practical interventions to enhance anemia prevention practices among adolescent girls.