Malaria is a third leading killer in Kenya and number one cause of morbidity and mortality in Kisumu East Sub-County (KESC) among under-fives. Study assessed levels of malaria awareness and knowledge on signs and symptoms among mothers. This was a cross sectional survey with 384 mothers, 2 health providers and 4 chiefs responders. KESC was purposively selected, 4 locations randomly sampled and mothers enrolled into the study by snowball sampling. Data was collected by Semi-structured questionnaires and Interview guides. Analysis was by descriptive and inferential statistics with significance level set at 0.05. From the results, approximately 383(99%) mothers knew mosquitoes transmit malaria, 378(98%) knew mosquitoes infected by malaria parasites cause the disease while 300(78%) knew mosquitoes breed in stagnant waters or swampy places. Hypothesis findings (p=0.023). Moreover, up to 99% of them knew malaria signs and symptoms. Further, 380(100%) recognized vomiting, 378(99%) said fever, 374(98%) knew appetite loss and 102(27%) headache as malaria signs in children. Similarly, 378(99%) knew inability to eat, drink or breast feed, 378(99%) recognized high body temperature, 268(71%) convulsion while 254(64%) mentioned vomiting as severe malaria signs in children. Additionally, 357(93%) mentioned headache, 339(88%) said fever, 333(87%) loss of appetite and 283(74%) joint pain, backache and body weakness as signs in pregnancy. Hypothesis showed, (p=0.039). Based on the results, it is concluded that most mothers knew malaria related information including signs and symptoms despite misconceptions. Therefore, there should be continuous health education through Radio stations, television and us of mobile telephony. There was also significant associations between levels of malaria awareness, signs and symptoms and malaria prevention.