Introduction: In our study, we aimed to investigate anatomy of testicular artery in cattle and ram by plastic injection and corrosion cast method. Materials and Methods: In our study, cattle and rams were used. The testes were cleaned, paying attention to the vascular and parenchymal structure. Then testicular artery was cannulated and prepared for plastic injection. Polyester was injected into the testiculary artery. The testes were kept at room temperature for 48-72 hours polyester in the vessel. The obtained corrosion casts were examined macroscopically. Results and Conclusions: When the course of arteria testicularis is examined macroscopically; on the surface of tunica albuginea arteria testicularis was found to divide 80%, in cattle and 60% in the rams. In general, in the corrosion casts of both species, the arterial testicularis showed very convoluted distribution in the funiculus spermaticus. In both species, when the arteria testicularis reaches the extremitas inferior of the arterial testis, two main branches are separated. These branches were observed to form a vascular network on the surface of tunica albuginea. As a result; although the course of the testicular artery in both species is similar, there are differences.