Salmonella is an important bacteria that can lead to infections both in the body and intestines, posing serious health concerns. This study looks at how Salmonella infection affects the body and immune system in BALB/c mice, focusing on immune reactions, antibody creation. Thirty mice were divided into three categories: a high-dose infection group, a low-dose infection group, and a control group. We measured antibody levels using ELISA, checked white blood cell (WBC) counts, and looked at liver and spleen tissues closely. The results showed changes based on the dose, such as higher antibody levels, more WBC counts, and significant tissue harm in those infected. Statistical tests showed clear differences among the groups, providing important information on how Salmonella operates and how the immune system responds. This research helps us learn about interactions between the host and the pathogen and lays the groundwork for creating specific treatment methods. This study helps in knowing how hosts and pathogens interact and lays groundwork for creating focused treatment methods.