Clostridium perfringens is an important pathogen in poultry, causing tuberculosis in broilers. Accurate diagnosis is essential for effective disease management. This study evaluated bacterial culture and PCR methods for the detection of C. perfringens, and correlated the results with the clinical manifestations of necrotic enteritis the alpha-toxin gene was targeted using conventional bacterial culture and PCR so tested fifty samples from chick sheep. Statistical analyzes were performed to compare the detection rates and to assess their relationship with clinical characteristics. Agarose gel electrophoresis was used to clarify the PCR results. Bacterial culture detected C. perfringens in 28 of 50 samples (56%), whereas PCR detected the pathogen in 42 samples (84%). The difference was statistically significant (χ2 = 11.245, p = 0.0214). A strong positive correlation (r = 0.78, p < 0.001) was observed between PCR-diagnosis and expression of clinical signs of necrotic enteritis Gel electrophoresis verified the 324 bp PCR product for the alpha-toxin gene. PCR showed higher sensitivity than bacterial culture for the detection of C. perfringens in flocks of poultry. The strong correlation between PCR findings and clinical manifestations suggests a potential increased risk of necrotic enteritis these findings recommend the use of PCR-based diagnostic technologies about in standardized surveillance programs to improve disease control in poultry production.