Objective: The gram-negative bacterium Vibrio cholera is the cause of the acute infectious bacterial illness cholera. It often spreads via ingesting contaminated food or water. Diarrhea and severe dehydration are symptoms of cholera. It can be lethal within hours if untreated. A fresh cholera outbreak struck many Iraqi cities in 2022 based on the World Health Organization's verified data. Methodology: A total 32 instances in all were recorded in the Medical City in Baghdad between June and November 2022, with the causal agent being found in stool samples from individuals who had been diagnosed as being infected. Distribution of the collected data is based on age and gender. Additionally, the isolated bacteria were tested for susceptibility to a variety of antibiotics, including Ciprofloxacin, Tetracycline, Ampicillin, Trimethoprim, and Supremethoprim. Statistics were applied to the obtained data. Results: Male were shown to have 55% more infections than female, who had 45%. Regarding demographics, it mostly targets the 22–42 age bracket. The greatest instances of cholera occurred in July and August, with the infection rising in the summer more than in the autumn. Statistics revealed that each antibiotic's sensitivity and resistance regardless of the patient's age and gender. The bacterial isolates were sensitive to Ciprofloxacin and Tetracycline. And resistant to other tested antibiotics. Conclusion: The antibiotic sensitivity of cholera was regardless to age and gender. Further studies needed to study the disease distribution according to geographical area in Baghdad as well as study the medical profile of infected people. Study sensitivity of the infected bacteria to other recent antibiotics is also recommended.