Plantago major leaves were used in traditional medicine for treatment many diseases; this research was performed out to qualitative investigation of phytocomponent in the Interested herb and also studies the antibacterial activity against certain types of bacterial isolated from clinical samples. The powder of plant leave was extracted with water/ ethanol (1:1). Following a qualitative screening for phytocomponents, the crude extract revealed the presence of several families of chemicals, including saponins, glycosides, phenols, tannins, steroids, alkaloids, flavonoids, and terpenoids. Three concentrations of crude extract were tested against five bacteria species include gram negative and gram positive (staphylococcus aurous, streptococcus pyogenous, pseudomonas, proteus spp.) using paper disc diffusion method. The three concentrations of crude extract was active in different percentage against all the tested bacteria.