Objective: The aim of the study was to assess the relationship between obesity measured by anthropometric measurements, vitamin D levels, and inflammatory status in Al-Ramadi obese adolescents. Methods: Between April and September of 2024, this study was carried out in the Maternity and Children Teaching Hospital in Ramadi, Iraq. A total of 60 teenagers were chosen, 30 of whom were obese (14 females and 16 males), and 30 of whom were normal weight as a control group. The InBody Device was used to evaluate anthropometric parameters such as body mass index (BMI), body fat percentage (BFP), skeletal muscle mass (SMM), waist-hip fat ratio (WHR), visceral fat, body fat, and basal metabolism. Insulin resistance (IR) and vitamin D levels were assessed using the linked enzyme immunoassay (ELISA) method. The Cobas e411 equipment was used to measure the levels of inflammatory cytokines, total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL). Results: Results showed significantly (P≤0.05) increases in anthropometric measurements, insulin resistance and pro-inflammatory cytokines (IL-6, TNF-α and IL-1β), whereas vitamin D levels showed significantly (P≤0.05) decreases in obese adolescents male and female than adolescents with normal weight male and female. Conclusion: The study concluded that the decreased in vitamin D levels appeared to be associated with obesity, as well as the high levels of insulin resistance (IR), inflammatory mediators and lipid profile that associated with obesity.