Abstract: Background and Aims: An important enzyme for dental health, alkaline phosphatase (ALP) serves a variety of purposes in preserving the structure and functionality of oral tissues. In order to better understand salivary ALP's potential as a biomarker for dental erosion and its diagnostic and prognostic utility in both young and adult populations, this study set out to clarify its possible role. Materials and Methods: In the study, there were twenty-seven patients with dental erosion and an equal number of age and gender-matched healthy controls. Before a meal and without stimulation, samples were taken from the control and study groups by passively drooling into a sterile glass tube until 5 ml was collected. Results: Salivary ALP levels showed a significant difference between patients with and without dental erosion (63.68 ± 4.36 (IU/L) versus 21.47 ± 1.16 (IU/L)), with a p-value of 0.0001 indicating a very high significance. Alkaline phosphatase enzyme levels and teeth erosion were found to have sturdy positive relation (r= 0.989) high significantly P< 0.001. Conclusion: Salivary alkaline phosphatase is a vital enzyme, which is essential for the mineralization of teeth and it considers biomarker for the severity and progression of dental erosion.