Abstract: Salmonella enterica is a major zoonotic pathogen responsible for foodborne illnesses worldwide. In this study, we aimed to identify S. enterica isolates from 200 clinical and environmental samples using both traditional microbiological techniques and molecular methods. Of the 100 bacterial isolates, 20 were confirmed as S. enterica through biochemical tests and molecular identification. Polymerase chain reaction (PCR) was employed to amplify the 16S rRNA, B-scDNAp, and Hd genes for species confirmation and characterization. All 20 isolates tested positive for the 16S rRNA gene, while 85% and 75% of the isolates were positive for B-scDNAp and Hd genes, respectively. The results highlight the effectiveness of using multiple molecular markers for accurate detection and strain differentiation in S. enterica. This approach enhances diagnostic precision and contributes to the understanding of the genetic diversity and potential virulence factors of S. enterica, offering valuable insights for public health surveillance and control strategies.