Toxocariasis is a parasitic zoonosis with worldwide distribution that affects both cats and dogs. Caused by Toxocara spp. Is a globally prevalent parasitic roundworm. It is a member of the Ascarididae family, which includes one of the most common intestinal parasites. The aim of this study was to investigate the prevalence of Toxocara spp. in a total of 150 cats fecal sample and eggs of T. spp in different stages development were incubated with Potassium Dichromate K2Cr2O7 extract in different concentrations 2.5% and 5% in vitro from two major breeds from Babylon (75 pets cats and 75 stray cats breeds). Therefore, this is the first parasite investigation as well as molecular characteristic analysis to be conducted in Babylon on pets and stray cats. After conducting a comprehensive examination, the clinical indicators exhibited by these animals were reported. In order to examine parasite eggs under a microscope, we collected feces from each animal. A small sample of faeces was also subjected to molecular analysis. Our investigation found that infestation rates, according to the microscopic method, were 24.6% (21% in stray and 16 % in pets cats). Microscopic investigations of the eggs incubated with Potassium dichromate extract demonstrated restricted larval growth at doses of 2.5% for 14 days but in concentaration 5% eggs developed faster to larvae L3 s, Subsequently, stray cats residing in hilla exhibit an elevated prevalence of Toxocara.spp. Therefore, it is crucial to develop effective methods or identifying and eliminating T.spp parasites in pet and straycats, while simultaneously prioritising public education on animal and human health. The findings highlight the imperative of preventive measures against toxocariasis due to its widespread occurrence. Recognising the inter connectedness of animal, environmental, and human health underscores the importance of deworming cats, promoting hygiene, and educating the public to mitigate the risks of this zoonotic condition. Protecting feline health benefits cats and reduces the likelihood of human transmission, creating a positive outcome for both.