Background: Toxoplasma is a parasitic illness that befalls a person through the consumption of food of Watchman characteristically meats that have cysts of T. gondii or via contact with the feaces of infected cats or a pregnant woman to the fetus during pregnancy. This study was aimed at assessing the burden of toxoplasmosis in varying geographical locations and enhancing the detection of T. gondii. Material and Methods: A new study was conducted in Babylon province Thursday (Jan 1, 2023- May 15, 2023) in Teaching hospitals with a sample size of 261 pregnant women and their paired cord blood to be screened of toxoplasmosis. A conventional PCR analysis at a molecular level was conducted on seropositive samples. Such diagnostic tests as MRIs or CT scans were applied with a view to diagnose brain lesions in extreme situations. Results: Also, anti-toxoplasma antibodies were desired, as well as DNA extraction through the help of extraction kits. Among the samples of maternal blood (87) there were toxoplasmosis-positive cases. Out of 40 cord bloods 38 percent were positive to the anti-Toxoplasma IgG, 3 percent positive to IgM, (15. 3%). Molecular screening using PCR revealed that all the samples to be positive to B1 gene (100%), 20 of the samples contained the P30 gene (50%) and 10 samples contained the 18Rdna gene (25%). Seropositive Sera 1) a sample of cord blood was positive and therefore, one new-born was proven to have congenital toxoplasmosis. Therefore, core relationship between relative parasite transmission risk and neonatal seropositivity was nowhere to be found (chi-square > 0. 05). Conclusion: This study comes clear about the fact that congenital toxoplasmosis posed itself more in the rural settlements than in the urban establishment. In terms of mitigating the risk of disease, it implies screenings early in life, that is, when a child is born and additional testing.