This study conducted at the College of Applied Medical Sciences /University of Kerbala focused on isolating and diagnosing bacteria from urine and tonsil samples collected from 50 students. The participants consisted of 25 females and 25 males, with ages ranging from 18 to 32 years. The samples were obtained within the university premises between December 20th, 2022, and March 25th, 2023. Standard culture media, including MacConkey agar, Blood agar, and chocolate agar, were utilized for direct inoculation of the samples. The cultures were incubated at 37 C for 24-48 hrs. Additionally, Muller Hinton agar was used for the Antimicrobial Susceptibility Test (Kirby-Bauer Disc Technique). Manual biochemical tests, such as Gram stain, Oxidase, Catalase, Urease, Coagulase, Citrate, and Triple Sugar Iron (TSI), were carried out for bacterial identification. The bacteria isolates were identified according biochemical tests into, namely Escherichia coli (4), Streptococcus pyogenes (4), Staphylococcus aureus (13), Klebsiella pneumoniae (3), Haemophilus influenzae (7), Staphylococcus saprophyticus (4), Acinetobacter baumannii (1), Neisseria gonorrhoeae (1), Streptococcus agalactiae (1), Enterococcus faecalis (1), and Staphylococcus epidermidis (1).