Background: The health-promoting effects of plant-derived natural products have long been studied in depth. The aim of this study is to evaluate the impact of palm heart extract (PHE) on lipid profile and other heart, liver, and reproductive system parameters in male rabbits. Methods: Four categories of male rabbits were selected and randomly assigned; three received a PHE-enriched diet and the fourth was fed with a regular diet for control purposes. The low, medium, and high PHE dose groups were each treated daily for 8 wk as in the chemical-induced group however; no treatment was given to control group. Liver function (ALT, AST, ALP and total bilirubin) and health indicators (troponin and lipid profile) were assessed with determination of levels. Also on several occasions during the study, the levels of testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH) were determined. Results: Lipid profile parameters, including cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL), and high density lipoprotein cholesterol (HDL), were assessed before and after the intervention period. Results: The results indicated a reduction in TC and LDL levels in the treatment group compared to the control group was statistically significant (p < 0.05). Nonetheless, there were no changes observed in the levels of TG and HDL in both groups, with a p-value greater than 0.05. These results indicate that heart palm water extract might have the ability to lower lipid levels, especially in decreasing total cholesterol and LDL levels. Testosterone levels showed a small drop in the high dose group when compared to the control group, although there was no statistical significance (p > 0.05). LH levels did not significantly decrease in the medium dose group compared to the control group (p < 0.05). There were no notable variations in FSH levels among the groups (p < 0.05). Conclusion: Male rabbits showed reduced troponin levels in a dose-dependent manner, indicating potential cardioprotective effects of HDP extract. Liver function was unaffected by the HDP extract administration. Even though levels of reproductive hormones varied, more study is needed to determine how heart palm extract affects male reproductive health.