Background: Nurses working in rural areas such as Osmanabad experience prolonged occupational stress owing to fewer staff, poor infrastructure, and emotionally charged clinical settings. Long-term exposure to pressures results in burnout, decreased psychological resilience, and suboptimal patient care. Although there is increasing awareness of nurse stress across the world, nurses in rural India have yet to benefit from mental health preventive programs. Objectives: This study was conducted to measure the effectiveness of an 8-week Mindfulness-Based Stress Reduction (MBSR) program among nurses in Osmanabad. Aim: The objectives are to examine the changes in perceived stress, burnout, resilience, and quality of sleep; and to explore nurses' experiences and barriers towards the practice of mindfulness. Methods: A mixed-method study, including 80 registered nurses working in Government hospitals and primary health centres, Osmanabad. Standard instruments were used to measure quantitative variables, such as the perceived stress scale (PSS), the Maslach burnout inventory (MBI), and the Connor-Davidson resilience scale (CD-RISC). Qualitative data were collected by semi-structured interviews with 20 participants after the interventions. Results were analysed with SPSS as well as a thematic analysis. Results: The statistical analysis showed a reduction in stress from 34.2% and a reduction in burnout indicators by 35.8% after the intervention. Resilience scores grew more than 20% and sleep quality was substantially better. Thematic analysis suggested that the intervention was associated with emotional grounding, increased self-compassion, and improved communication. Conclusions: It was feasible and effective to practice mindfulness in this resource-limited context of Osmanabad. The intervention resulted in demonstrable psychological benefits and promoted a generation of more compassionate and resilient nursing staff. This study further recommends that MBSR be incorporated into rural health policy and nursing education to protect the mental health of frontline caregivers.