Gallstone disease (GD) is a common disorder worldwide. That Affecting variable age and gender categories, most of them are female gender (about 2-3 times higher than male). The influence of female sex hormones on the development of gallstones is well established. Therefore, the study focused on the extent of the relationship between gallstone disease and female hormones. The study included (100) Iraqi female patients with gallstone disease, aged (18±65) years. These female patients were referred to the Department of Immunology/ Medical City in Baghdad province/ Iraq during the time period (from Oct 2024 to Jan 2025). We concluded that the gallstone disease hormones tests in the included sample of female patients was high than the controls, Accordingly Incidence of gallstones disease was positively correlated to Gender and Sex hormones. Our aim to make a clear insight about the relationship between different female hormones and thyroxin to determine the dangerous factors associated with progression of gallstones.