Since bioremediation of pesticides is environmentally safe, economical, and efficient, it is now the preferred option. The purpose of the study was to assess the Pseudomonas sp. Bioremediation Chemosate at various concentrations (10,20,30) ppm and incubation times. The isolation of the Pseudomonas sp from the soil region in Baghdad grows on Pseudomonas selective at room conditions for (18-48) hours at 35 Ċ. Pseudomonas sp. are the bacteria that are produced when soil samples are diluted using selective media. Because glycerol was added to the culture as a reagent, isolated Pseudomonas aeruginosa produced a strong strain with green-blue. It was then grown on mineral salt media as a source to examine the biodegradation for (10,20,30) ppm during (10,20,30) days to count the bacterial growth. The best results were 20 ppm/0.729, 20 days incubation, while the best Pseudomonas areuginosa degradation rate% was 10 ppm/97.5%, 10 days. The HPLC results showed that 10 ppm at 10 days produced the optimum bio-degradation results.