The research work presented in my thesis consists of Design & Development of Benzimidazole Derivatives for Anti-Inflammatory Activity. Benzimidazole derivatives have been shown to have wide variety of pharmacological activities like Anti helminthic, Anti-inflammatory Diabetes, Cancer. The effective synthesis and detailed characterization of series of thiazolidinedione derivatives using infrared (IR), nuclear magnetic resonance (NMR), and mass spectrometry (HRMS). The synthesis was carried out by a multi-step synthetic process that began with starting material Benzimidazole. A combination of spectroscopic methods was used to characterize the synthesized Benzimidazole derivatives. IR spectroscopy gave crucial information on the functional groups present in the compounds, allowing the Benzimidazole-one core structure to be confirmed as well as the presence of additional substituents. The structural connectivity and stereochemistry of derivatives were determined using NMR spectroscopy (1H). Molecular docking studies were carried out to assess the binding affinities of the synthesized Benzimidazole derivatives to inflammation such as Cyclin dependent kinase 1/2/4/6, PPAR- Ƴ and 15-PGDH, tubulin. When compared to the co-crystallized ligand, the selected four compounds showed highest binding energy. This indicates that they have the potential to be effective inhibitors of these biomarkers. For the Biological activity (anti-inflammatory) performed at results showed that Compound 2- (6-bromo-1-ethyl-1H-benzo[d]imidazol-2-yl) acetic acid showed better anti-inflammatory activity (protein denaturation method). Compound A (6-chloro-1-ethyl- 1H-benzo[d]imidazol-2-yl) acetic acid, B 2-(6-bromo-1H-benzo[d]imidazol-2-yl) acetic acid & C (6-chloro-1-methyl-1H-benzo[d]imidazol-2-yl) acetic acid showed lowest anti- inflammatory activity when compared to positive control.