The present research was performed using animals at the College of Veterinary Medicine, Tikrit University during the period between September 1, 2025, and December 1, 2025, in the animal house of the college under standard laboratory conditions of rearing animals. This study used male laboratory rats. The males were selected because they are larger and having a more stable weight than the females hence reducing hormonal interference that would have influenced the outcome of the study. At the beginning of the experiment, the weight of the rats was 250 to 300 grams. Pre-weighing of the animals was done prior to feeding to find out the initial weight of each rat. Monitoring was done over the 60 day period of rearing whereby under appropriate environment they received free access to water and feed. ELISA was used to measure liver enzymes (ALT, AST, and ALP), total cholesterol and triglycerides in serum, according to the laboratory standards. The levels of GOT (AST) and GPT (ALT) were significantly raised at the end of the feeding period, which means that the cells of the liver were damaged by the fat accumulation inside of the cells and increased the cell membrane permeability. Conversely, the level of the enzyme alkaline phosphatase (ALP) did not show any significant change, which means that the pathological effect of obesity was not serious at the initial stages and did not spread to the bile ducts. The findings also presented the fact that the total cholesterol and triglyceride levels had been significantly increased. It may be concluded that dietary induced obesity is one of the key causes of lipid imbalance and hepatocellular damages, and can be one of the initial phases in the evolution of metabolic liver diseases in the event that exposure to causative factors is sustained.