Background: It has been proven that residential environment and biological sex affect immunity, but there is limited evidence of the combined effect of both factors on immunoglobulins in serum of university students. Objectives: To examine (IgG, IgA, and IgM) concentrations in serum of undergraduate students depending on residential status, gender, and years of dormitory living. Methods: The cross-sectional study included 60 undergraduate students who were further stratified by gender (30 males, 30 females) from the Southern Technical University, College of Health and Medical Technologies. There were 40 dormitory students and 20 non-dormitory students enrolled in the study. Serum immunoglobulins concentration was measured through automated Abbott Architect Ci4100 analyzer. Results: Significantly (p≤0.05) increased levels of serum IgG were noted in dormitory residents (1273.37±218.77) mg/dL compared to non-dormitory residents (963.07±262.32) mg/dL according to gender among dormitory residents. The present data showed that female students had significantly higher (p≤0.05) levels of IgM (152.22±67.954) mg/dL compared to male 94.25±44.22 mg/dL, while IgA levels in male showed a significant elevation (p≤0.05) in male (242.75±84.92) mg/dL compared to female (192.37±49.697) mg/dL. Regarding to the duration of residence of students less than 2 years group showed higher concentrations of IgM (172.52±61.87) mg/dL compared to more than 2 years group (137.56±58.825) mg/dL. While IgG exhibited a significant increase in more than 2 years group (1304.36±199.09) mg/dL compared to less than 2 years group (1172.76±233.73) mg/dL. Conclusion: The present study demonstrated that dormitory residence was associated with altered humoral immune responses among university students particularly through elevated serum IgG concentrations compared with non-dormitory.