Background: In recent years, green nanotechnology has proven to be as a viable way to create metallic nanoparticles with the use of phytochemicals. Objective: This paper aims to explore bacterial synthesis of silver nanoparticles (AgNPs) with plant extracts of Angelica glauca and Paeonia suffruticosa and characterize their properties, structure, antimicrobial activity, and anticancer. Methodology: The silver nanoparticles were synthesized through plant-based reduction of silver nitrate and characterized by several technologies. Agar-well diffusion assay was used in order to determine the antimicrobial activity whereas the anticancer activity on human breast cancer cells (MCF-7) was evaluated. Results: GC-MS study of Paeonia suffruticosa extract identified several of bioactive phytochemicals. The findings revealed the change of color of light yellow to dark brown, which indicated the creation of silver nanoparticles. Spectrophotometric analysis indicated the formation of these nanoparticles as it yielded the absorption peak at around 420-440 nm. The existence of intense, broad (FTIR) peaks was a good testament to the existence of hydroxyl (OH) groups as well. The SEM and TEM obtained results indicated that a majority of the synthesized nanoparticles had an angular spherical shape and was between the 30 and 98 nm sizes. Plant-based silver nanoparticles have demonstrated antibacterial effects superior to crude extracts on Gram-positive and Gram-negative bacteria, in bioactivity assays. Conclusion: Silver nanoparticles prepared with extracts of Angelica glauca and Paeonia suffruticosa roots are attractive multifunctional nanomaterials with strong antimicrobial and anticancer activities. Their characteristics indicate that they are promising to biomedical and pharmaceutical applications in the future.