Background: Exposure to ionizing radiations at work is an inevitable aspect of contemporary medical practice, especially in radiology units where radiology staff routinely operate with diagnostic imaging equipment like X-ray units. Aim: The present study aimed to determine the impact of X-ray radiations on X-ray technicians by measuring the levels of (CRP) C-reactive protein and (IL-6) Interleukin-6. Methodology: This study included a total of 75 male subjects. The study population comprised 50 X-ray technicians chosen randomly among the radiology departments in hospitals in Basrah province, Iraq. Concentrations of Serum CRP were measured by Genrui PA54 biotech kit and ELISA was used to quantify IL-6 concentrations. Results: The study showed that median serum IL-6 concentration was significantly higher (p≤0.05) in X-ray technicians 3.48 pg/mL (IQR:1.50-5.2) compared to control 1.10 pg/mL (IQR:1.10-1.50) as well as, the present results exhibited a significant increasing (p≤0.05) in the concentrations of CRP among X-ray workers 1.81 mg/L (IQR:1.31-4.10) compared to control (non-exposed) 0.91 mg/L (IQR:0.50-2.44) However, according to the years of work. The current data showed a significant increase (p≤0.05) in the concentrations of IL-6 in more than10 years group 3.49 pg/mL (IQR:1.50-5.2) compared to group 1 (less than10 years 1.49 pg/mL (IQR:1.10-3.49) while CRP didn’t show any significant differences. Conclusion: Chronic occupational exposure to ionizing radiation was associated with increased circulating levels of IL-6 and CRP among X-ray technicians. The significant elevation of IL-6 in workers with long exposure duration suggests that cumulative radiation exposure may promote sustained inflammatory activation.