Background: One of the major global health problem and oxidative stress has an important role in its pathogenesis is kidney injury. Researchers showing that chia seeds (Salvia hispanica) contain high levels of antioxidants, omega-3 fatty acids, and bioactive compounds that can works as nephroprotective agents. The aim of this study to evaluation of the protective effect of chia seed extract against gentamicin-induced nephrotoxicity in male tats. Methods: A total of forty male Wistar rats (200–250g) were assigned to five groups (n = 8). Group 1 (Control); Group 2 (Gentamicin, 100 mg/kg); Group 3 (Gentamicin + Low-dose chia extract,)200 mg/kg); Group 4 Gentamicin + Medium-dose chia extract,) 400 mg/kg) and Group 5 Gentamicin + High-dose-chia-extract,)600 mg kg [24]. Oral administration of treatments was done for 10 days. Biochemical parameters serum creatinine, blood urea nitrogen (BUN), uric acid also antioxidant markers (SOD, CAT, GPx and MDA) were determined. Results: An administration of gentamicin proved significantly higher serum creatinine (2.45±0.28 mg/dL), BUN (68.34±5.12 mg/dL), and uric acid levels compared to controls (p< 0·001). The treatment with Chia extract was associated to a significant improvement in all these parameters, being more evident for the high-dose group (creatinine: 1.23±0.18 mg/dL; BUN: 35.67±3.89 mg/dL). Chia-treated groups showed strong restoration of activities of the antioxidant enzymes (SOD, CAT and GPx), compared to untreated counterparts, in association with decreased MDA levels. Conclusion: Chia seed extract protected the kidney from gentamicin-induced renal injury via antioxidant mechanisms and preservation of renal function. This result indicates the possible therapeutic usage of chia seeds in preventing drug-induced nephrotoxicity.