Polycystic ovarian syndrome (PCOS), a prevalent endocrine and metabolic condition, is frequently considered a principal cause of anovulatory infertility in women. The pathophysiology of PCOS is intricate and affected by various factors. Recent research indicates that disruption in energy metabolism and oxidative stress are critical factors in abnormal follicular growth and reduced fertility in people with PCOS. The present study aimed to evaluated level of glutathione, catalase, malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8- OHdG) in PCOS women. The study had a total of 90 samples, with 60 of them being diagnosed with PCOS and the remaining 30 serving as controls. The age range of the participants was between 20 and 45 years. The patients were admitted to both Tikrit Teaching Hospital and a private hospital in Tikrit. The present study demonstrated decrease level of glutathione and catalase in PCOS as compared with control at p-value < 0.05. While increase level of MDA and 8-OHdG in PCOs as compared with control, at p-value < 0.05. Conclusion: The current investigation has shown that serum glutathione and catalase levels were reduced in women with PCOS. Patients with polycystic ovary syndrome exhibit elevated oxidative stress.