This study identified isolates of Staphylococcus aureus, obtained the antibiotic susceptibility pattern of MRSA isolates and also determined the prevalence of MRSA strains from various wound samples collected from patients in Kano central, Kano State. Identification of Staphylococcus species in the present study was based on Gram staining, cultural characteristics and biochemical characterization. The isolates were positive for catalase, coagulase and DNase test. The findings revealed that out of 217 total samples tested, S. aureus was detected in 77 (35.48%), while Methicillin resistance Staphylococcus aureus (MRSA) was present in only 4 (1.84%) of the cases. Among the hospitals, S. aureus prevalence was highest at Murtala Muhammad Specialist Hospital (38.89%), followed by National Orthopedic Hospital Dala (42.59%), and lowest at Dawakin Kudu General Hospital (26.03%). Further investigation into antibiotic resistance patterns showed varying levels of resistance among the tested isolates showed highest resistance was observed against Amoxicillin (50.65%), Chloramphenicol (46.75%), and Ciprofloxacin (44.16%), while Oxacillin and Cefoxitin (5.19%) had the lowest resistance, which aligns with their role in MRSA screening. Molecular analysis for methicillin resistance genes (mecA and mecC) in the present study revealed that 50% of tested isolates carried the mecA gene, while 25% harbored mecC, and no isolates contained both genes simultaneously.