The study of atmospheric pollen content of an area is useful in providing vital information on environmental change, vegetation type, species composition and their utilization for safety health and sustainable development. The current study examined the diversity, morphology and comparative study of airborne pollen grains from four Local Government Areas in Akoko division, Ondo State, Nigeria. This study was carried out in fourty randomly selected locations to examine the temporal and spatial relationship between the atmospheric pollen content and its vegetation. The airborne pollen grains were collected with Modified Tauber Sampler using simple random sampling technique and analyzed palynologically. Results showed 50,661 pollen grains comprising of 182 pollen types belonging to 74 angiospermic plant families were encountered. Three (3) of these pollen types were identified to family level, 57 to species level, 121 to generic level and one (1) unidentified. The predominant pollen types belong to the family Poaceae, Elaeis guineensis, Alchornea cordifolia, Aspilia africana, Casuarina equisetifolia, and Tridax procumbens. The presence of Empetrum nigrum, Linnea borealis, Linum sp., Stereospermum kunthianum and Vitellaria paradoxa pollen indicates evidence of long distance transport. Airborne pollen grains were most abundant between September and January. Rainfall and relative humidity had more negative effect on pollen concentration. The effects of temperature and light intensity varied considerably with time and location because their effects are influenced by other meteorological factors. Continuous environmental monitoring through pollen rain analysis is recommended in order to produce a pollen calendar for the studied area.