The development of sea transportation has led to an increase in the intensity of ship use both for people, goods, and ship departure schedules. The volume of ship departure and arrival has an important role in the transmission of various kinds of insect pests that can potentially cause disease to the crew (ABK) and passengers, both between regions or across countries. The purpose of this study was to determine the factors associated with the presence of rats on passenger ships that dock at the Port of Kendari City. This type of research is analytic cross sectional design research. The population in this study is the local passenger ship that docked at the Port in the city of Kendari as many as 44 ships. The sample size of 40 ships was selected using simple random sampling technique. Measurement of temperature and humidity using a hygrothermometer while lighting using a lux meter. Data on sanitation conditions was collected using a marine sanitation checklist sheet. The collected data is then analyzed using the chi square test and the phi coefficient. The results showed that there was a relationship between temperature, humidity and lighting with the presence of rats on the ship, where the value of p <α = 0.05 was obtained. Periodic maintenance and cleaning efforts are needed for lighting and room maintenance facilities so that the temperature, humidity and lighting in the room always meet the requirements and can reduce the potential for rat activity on the ship.