Background and purpose: Staphylococcus aureus is a common pathogen of nosocomial infections. Increased infections caused by this bacterium in developing countries have led to many problems. The aim of this study was to determine the prevalence of antibiotic resistance of S. aureus isolated from urinary tract infections to some commonly used antibiotics. Materials and Methods: In this cross-sectional descriptive study, 100 S. aureus isolates were collected from patients with urinary tract infections. These isolates were identified using standard laboratory and culture methods in a specific medium. Kirby-Bauer disk diffusion method was used to evaluate the antibiotic susceptibility pattern of the strains. Results: Phenotypic evaluation of antibiotic resistance pattern of S. aureus strains showed the highest resistance to antibiotics methicillin and penicillin (91%), erythromycin (86%) and gentamicin (75%) and the highest sensitivity was related to The antibiotics were nitrofurantoin (95%) and vancomycin (86%). Conclusions: The present study showed increased resistance to different antibiotics by S. aureus, which is a serious warning for treatment of S. aureus infections in the area. Therefore, in order to prevent increased resistance to other antibiotics, it is necessary to refrain from unnecessary administration of available antibiotics.