The quality of health services is the degree of perfection of health services in accordance with professional standards and service standards using the potential resources available in hospitals or health centers in a reasonable, efficient, and effective manner. This study aims to determine the effect of service quality between perceptions and expectations on the quality of post-accreditation outpatient health care services in sulewana puskesmas (Community Health Center) Poso Regency. This study uses quantitative research methods with a cross-sectional study design. The population in this study were 25 health workers who provided health services in the outpatient unit of the sulewana puskesmas and all outpatients who came to visit the puskesmas of Sulewana were 6,526 in 2019. The sampling technique was done by accidental sampling. Data were collected through direct interviews using a research instrument in the form of a questionnaire. Data were analyzed univariately and bivariate. The results showed that there was an influence of physical evidence (p = 0.003), reliability (p = 0.004), and collateral (p = 0.022) on the quality of post-accreditation outpatient health services in the sulewana health center in Poso Regency. It is expected that the health workers so that the procedure for admitting outpatients in the administration section of the Puskesmas Sulewana, Poso Regency will not be complicated (fast) and to further improve the quality of health services towards plenary accreditation, by further improving the quality of the tangibles dimension which impacts on chapter II, dimensions The reliability of impact in chapter I, and the assurance dimension impact on chapter III of the puskesmas accreditation standards.