Cervical cancer is the fourth most frequent cancer in women with an estimated 570,000 new cases in 2018 representing 6.6% of all female cancers. Cervical cancer is the leading cause of cancer death in women in low-HDI countries. Cervical cancer ranks first among the female cancer in Nepal with age-adjusted incidence rate 21.5, 2,942 new cases and 1,928 deaths. The high mortality rate from cervical cancer globally could be reduced through prevention, early diagnosis, effective screening and treatment. The study aim to identify the knowledge and practice of cervical cancer screening among women attending gynecology OPD. A descriptive cross-sectional study design was used in this study. Hundred and fifty women were selected through purposive sampling technique. A pretested semi structured interview schedule was used to measure research variables. Frequency, percentage, mean and chi-squired test were used to analyze the data. Out of 150 women, mean age was 39.41 ± 10.26 and 78.7% respondents were Hindu. More than three fourth (84.7%) were literate. Slightly more than one third (34.7%) of the respondents herd about cervical cancer screening. Among them, less than half (46.2%) had adequate knowledge and only 8.7% of women had practice of cervical screening. There were no association between knowledge and socio-demographic variables but the practice were associated with residence (p=<0.05) and family income (p=<0.05. A majority of women have inadequate knowledge and low practice of cervical cancer screening. Therefore cervical cancer screening and awareness program should be emphasized.