Flying Fish is an important fishery source in the tropics and sub-tropics because of its high economic value, so that exploitation continues to be carried out even some waters in Indonesia have experienced high fishing pressures. This study aims to identify the biological factors of Flying Fish (Decapterus spp) that catches purse seine in the waters of the South Buton Regency. The method of collecting data uses the method of observation (observation) of the catch of purse sein vessels every day for 3 (three) months. Analysis of growth parameters, mortality, length first captures, using RStudio software. From the analysis results obtained asymptotic length (L∞) of 27.10 cm and the value of growth rate (K) of 0.196, t0 of -, 0471. Total mortality was 0.311, natural mortality was 0.257, arrest mortality was 0.054, and the rate of exploitation was 0.175. The growth rate is relatively slow with a K value of 0.196. The exploitation rate value (E) has not exceeded the optimum value of 0.5 meaning that Flying Fish in South Buton waters is suspected to have a low exploitation rate and a high spawning potential. The length of the first gonad (Length of the first maturity) (Lm) at 14.2 cm, while the length of the first fish caught (Lc) at 16.6 cm, the optimal length (Lopt) of 18.3 cm. This indicates that generally Flying Fish caught in South Buton waters have matured.