Organochlorine pesticides (OCPs) are toxic environmental pollutants of worldwide concern, distributed in trace quantities within the aquatic ecosystem components (water, sediments and fish) and can possibly bio-concentrate, bio-accumulate, bio-transform and/or bio-magnify through the food web and constitute serious environmental and human health hazards. Hence, the need to determine and quantify the concentrations of OCPs in selected water bodies in some states in South-West Nigeria to ascertain the safety of the aquatic components especially the fishes which are for consumption. The study was carried out in Lagos, Oyo, Ogun and Ondo States, where two (2) water bodies in each of the State were purposively selected based on their popularity for fishing activities and other anthropogenic activities. The aquatic components (water, sediment and fish) samples were obtained, extracted and examined for OCPs forms (Dichlorodiphenyltrichloroethane [DDT], endosulfan and aldrin). The water samples retained mean concentrations (DDT [0.001 - 0.023 µg/ml], endosulfan [0.001 - 0.080 µg/ml] and aldrin [0.001 - 0.003 µg/ml]), sediment samples (DDT [0.003 - 0.028µg/g], endosulfan [0.002 - 0.355µg/g] and aldrin [0.002 - 0.013 µg/g]) and fish samples (DDT [0.001 - 0.016 µg/g], endosulfan [0.001 - 0.111 µg/g ] and aldrin [0.004 - 0.123 µg/g]). The detected OCPs concentrations except for endosulfan in the water and sediment components were within WHO set maximum residual limit (MRLs) while in fish samples, the residual concentrations except for aldrin were within set MRLs. DDT, endosulfan and aldrin are persistent in the environment and have been classified by NAFDAC as likely human carcinogens or carcinogenic agent, hence their prolong exposures in the aquatic environment may result in environmental degradation, loss of aquatic biodiversity and human health hazards.