This study reveals the distribution and levels of the organochlorine pesticide residues in fish, water and sediments in South-South, Nigeria. They were extracted with dichloromethane and screened for organochlorine pesticides with Gas Chromatography-Mass Spectrophotometry (GC-MS). All data collected were exposed to analysis of variance at (p<0.05) and the means were separated by means of Duncan Multiple Range Test (DMRT). It was discovered in this study that organochlorine pesticide residues were not discovered in all the fish and water sampled, while there was significant difference (P<0.05) in the levels of some organochlorine pesticides detected in the sediments. Sediment samples from Oron in Akwa-Ibom State recorded 1.50±0.25 µg/kg and 30.80±0.20 µg/kg for lindane and aldrin residues respectively; Mkpana Beach (Akwa-Ibom State) had lindane (3.70±0.20 µg/kg), aldrin (4.20±0.20 µg/kg), endosulfan (0.60±0.30 µg/kg) and ortho-para-dichloro-diphenyl-trichloro ethane (o,p’- DDT) (0.20±0.10 µg/kg). Illushin and Agenebode in Edo State had 0.10±0.02 µg/kg and 1.00±0.30 µg/kg of aldrin residues correspondingly. They were all above the allowable limit of 0.05 µg/kg recommended by Environmental Protection Agency (EPA). The pesticide-free fish and water could be as a result of the fact that the Fisher folks no longer use pesticides in fishing due to the awareness of the imminent dangers involved. However, the high levels of Pesticide residues in sediments may be as a result of overtime bioaccumulation of extensive and repeated use of the pesticides in fishing.